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total stat-5 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc total stat-5 antibody
    Total Stat 5 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/total stat-5 antibody/product/Cell Signaling Technology Inc
    Average 90 stars, based on 1 article reviews
    total stat-5 antibody - by Bioz Stars, 2026-03
    90/100 stars

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    Schematic of interleukin-20 receptor complex before and after activation by IL-20, -19, or -24. The type I receptor is composed of the interleukin-20RA (IL-20RA) and IL-20RB proteins while the type II receptor contains the IL-22RA1 and IL-20RB proteins [ , , ]. Upon cytokine binding, Jak1 binds near the transmembrane region of IL-20RA and IL-22RA1. Tyk2 is proposed to bind to IL-20RB based on sequence homology between IL-20RB and interferon-γ receptor 1 (IFNγR1) . Binding of the cytokine results in closer juxtaposition of Jak1 and Tyk2, leading to autophosphorylation of the tyrosine kinases, phosphorylation of IL-20RA, and recruitment and phosphorylation of signal transducer and activator of transcription (STAT)-3, STAT-1, and possibly, <t>STAT-5</t> [ , ]. The STATs can then translocate to the nucleus to activate transcription.
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    Schematic of interleukin-20 receptor complex before and after activation by IL-20, -19, or -24. The type I receptor is composed of the interleukin-20RA (IL-20RA) and IL-20RB proteins while the type II receptor contains the IL-22RA1 and IL-20RB proteins [ , , ]. Upon cytokine binding, Jak1 binds near the transmembrane region of IL-20RA and IL-22RA1. Tyk2 is proposed to bind to IL-20RB based on sequence homology between IL-20RB and interferon-γ receptor 1 (IFNγR1) . Binding of the cytokine results in closer juxtaposition of Jak1 and Tyk2, leading to autophosphorylation of the tyrosine kinases, phosphorylation of IL-20RA, and recruitment and phosphorylation of signal transducer and activator of transcription (STAT)-3, STAT-1, and possibly, STAT-5 [ , ]. The STATs can then translocate to the nucleus to activate transcription.

    Journal: Molecular Vision

    Article Title: Analysis of interleukin-20 receptor complexes in trabecular meshwork cells and effects of cytokine signaling in anterior segment perfusion culture

    doi:

    Figure Lengend Snippet: Schematic of interleukin-20 receptor complex before and after activation by IL-20, -19, or -24. The type I receptor is composed of the interleukin-20RA (IL-20RA) and IL-20RB proteins while the type II receptor contains the IL-22RA1 and IL-20RB proteins [ , , ]. Upon cytokine binding, Jak1 binds near the transmembrane region of IL-20RA and IL-22RA1. Tyk2 is proposed to bind to IL-20RB based on sequence homology between IL-20RB and interferon-γ receptor 1 (IFNγR1) . Binding of the cytokine results in closer juxtaposition of Jak1 and Tyk2, leading to autophosphorylation of the tyrosine kinases, phosphorylation of IL-20RA, and recruitment and phosphorylation of signal transducer and activator of transcription (STAT)-3, STAT-1, and possibly, STAT-5 [ , ]. The STATs can then translocate to the nucleus to activate transcription.

    Article Snippet: The membranes were then probed with the following signal transducer and activator of transcription (STAT) antibodies (all antibodies from Cell Signaling Technology Inc., Danvers, MA, unless stated otherwise): phospho-tyrosine701 STAT-1 (mouse monoclonal; ThermoFisher, 33–3400), total STAT-3 (mouse monoclonal; 9139), phospho-serine727 STAT-3 (rabbit polyclonal; 9134), phospho-serine705 STAT-3 (rabbit polyclonal; 9131), total STAT-5 (rabbit polyclonal; 25,656), and phospho-TyrosineY694 (mouse monoclonal; 9356).

    Techniques: Activation Assay, Binding Assay, Sequencing

    Western immunoblot analysis of STAT phosphorylation. Western immunoblot analysis of signal transducer and activator of transcription (STAT)-1 Tyr701, STAT-3 Ser727, STAT-3 Tyr705, and STAT-5 Tyr694 phosphorylation in ( A ) normal human trabecular meshwork cells or ( B ) normal human dermal fibroblasts treated with the indicated interleukins for 15 min. Total STATs were used as loading controls. The arrow points to the upper phosphorylated band. C: Densitometry of the phosphorylated Ser727 STAT-3 band. Trabecular meshwork, n=6 technical replicates using four independent cell strains; dermal fibroblasts, n=3 technical replicates from three cell strains. * p<0.05; ** p<0.005 with one-way ANOVA.

    Journal: Molecular Vision

    Article Title: Analysis of interleukin-20 receptor complexes in trabecular meshwork cells and effects of cytokine signaling in anterior segment perfusion culture

    doi:

    Figure Lengend Snippet: Western immunoblot analysis of STAT phosphorylation. Western immunoblot analysis of signal transducer and activator of transcription (STAT)-1 Tyr701, STAT-3 Ser727, STAT-3 Tyr705, and STAT-5 Tyr694 phosphorylation in ( A ) normal human trabecular meshwork cells or ( B ) normal human dermal fibroblasts treated with the indicated interleukins for 15 min. Total STATs were used as loading controls. The arrow points to the upper phosphorylated band. C: Densitometry of the phosphorylated Ser727 STAT-3 band. Trabecular meshwork, n=6 technical replicates using four independent cell strains; dermal fibroblasts, n=3 technical replicates from three cell strains. * p<0.05; ** p<0.005 with one-way ANOVA.

    Article Snippet: The membranes were then probed with the following signal transducer and activator of transcription (STAT) antibodies (all antibodies from Cell Signaling Technology Inc., Danvers, MA, unless stated otherwise): phospho-tyrosine701 STAT-1 (mouse monoclonal; ThermoFisher, 33–3400), total STAT-3 (mouse monoclonal; 9139), phospho-serine727 STAT-3 (rabbit polyclonal; 9134), phospho-serine705 STAT-3 (rabbit polyclonal; 9131), total STAT-5 (rabbit polyclonal; 25,656), and phospho-TyrosineY694 (mouse monoclonal; 9356).

    Techniques: Western Blot